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pAAV-hsyn-Jaws-KGC-GFP-ER2 in Rhesus macaque (AAV Retrograde)

Addgene #65014-AAVrg

Data Submitted By
Adriana Galvan
Submission Date
July 01, 2019
Publication Date
July 01, 2019 (modified May 16, 2023)
Abstract
Not Provided
Expression of Jaws on cortical neurons after injection of AAVretro-hSyn-Jaws-KGC-GFP-ER2 injection in the putamen of a rhesus macaque. The image on the right shows a higher magnification of an adjacent area. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. SMA, supplementary motor area (Galvan et al., unpublished data)
Caption: Expression of Jaws on cortical neurons after injection of AAVretro-hSyn-Jaws-KGC-GFP-ER2 injection in the putamen of a rhesus macaque. The image on the right shows a higher magnification of an adjacent area. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. SMA, supplementary motor area (Galvan et al., unpublished data)

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hsyn-Jaws-KGC-GFP-ER2
Addgene #65014-AAVrg

Virus & Injection

Virus

Virus Name
pAAV-hsyn-Jaws-KGC-GFP-ER2
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
65014-AAVrg
Promoter
Synapsin
Cargo Type
Optogenetics
Injection Titer / Dose
9.00E+12 GC/ml
Injection Volume
1 μl
Injection Rate
200 nl/min
Injector Material
Not Provided
Injection Site / Route
Putamen, NeuroNames ID: 230
Additional Details
Injected in putamen to target neurons projecting to this region.
View in Addgene Catalog

Other Details

Species
Non-Human Primate
Strain or Cell Line
Rhesus macaque
Source
Not provided
Age at Injection
5 years
Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Assay & Results
Strong expression of neuronal cell bodies in most areas known to project to putamen (various areas of cortex, amygdala, subthalamic nucleus, Gpe); with two major exceptions: intralaminar nuclei of the thalamus.
Functional Testing
The genetic tool (Jaws opsin) was not tested. Expression was only evaluated based on histology