pAAV-hsyn-Jaws-KGC-GFP-ER2 in Rhesus macaque (AAV Retrograde)
Addgene #65014-AAVrg

Expression of Jaws on cortical neurons after injection of AAVretro-hSyn-Jaws-KGC-GFP-ER2 injection in the putamen of a rhesus macaque.   The image on the right shows a higher magnification of an adjacent area. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. SMA, supplementary motor area (Galvan et al., unpublished data)

Caption: Expression of Jaws on cortical neurons after injection of AAVretro-hSyn-Jaws-KGC-GFP-ER2 injection in the putamen of a rhesus macaque. The image on the right shows a higher magnification of an adjacent area. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. SMA, supplementary motor area (Galvan et al., unpublished data)

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
9.00E+12 GC/ml
Injected Volume
1 μl
Injection Rate
0. 2 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Injected in putamen to target neurons projecting to this region
View in Addgene Catalog

Subject

Species / Strain
Rhesus macaque
Age at Injection
5 years

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Expression Site
Strong expression of neuronal cell bodies in most areas known to project to putamen (various areas of cortex, amygdala, subthalamic nucleus, Gpe); with two major exceptions: intralaminar nuclei of the thalamus and
Genetic Tool Outcome
The genetic tool (Jaws opsin) was not tested. Expression was only evaluated based on histology