pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Rat (AAV8)
Addgene #44361-AAV8

Confocal image, 20x magnification, 560nm excitation

Caption: Confocal image, 20x magnification, 560nm excitation

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.50E+13 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Ventral striatum - nucleus accumbens core
View in Addgene Catalog
Secondary Virus
CAV2-Cre
Injected Titer
2.50E+09 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum: Substantia nigra
Injection Site Comments
Since injection was at the same site, both viruses were mixed 1:1 ratio and then a total of 1. 2 μl was injected to give 600 nl of each virus. We waited 5 min before removing the injection pipette from the injection site

Subject

Species / Strain
Rat Outbred Sprague-Dawley
Stock Number
Envigo rat stock #002
Age at Injection
8 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Nucleus accumbens, direct pathway medium spiny neurons