pAAV-CAG-tdTomato (codon diversified) in Mouse (PHP.S)
Addgene #59462-PHP.S

tdTomato is in yellow; DAPI in blue. Imaged on a Leica SPE microscope with a 20x objective. 16 μm sections of the mouse olfactory epithelium

Caption: tdTomato is in yellow; DAPI in blue. Imaged on a Leica SPE microscope with a 20x objective. 16 μm sections of the mouse olfactory epithelium

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.00E+12 or 1.00E+13 GC/ml
Injected Volume
100 μl
Injection Rate
Via 0. 3 mL insulin syringe
Injection Site
Intravenous (retro-orbital or tail vein)
Injection Site Comments
Retro-orbital and tail vein inject to target sensory neurons, ideally those in the olfactory epithelium
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
8 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
In the olfactory epithelium, I observed very sparse expression in the sensory neurons and stronger expression in sustentacular cells
Other Information
No toxicity or inflammation. The number of cells infected in the 1e11 VG and 1e12 injections did not seem that different. In both cases the number of sensory neurons that were infected were quite low
Tips & Comments
In our experience, it seems that the AAV PHP. S variants do not offer much of an advantage over other more reliable methods of transducing olfactory sensory neurons, such as with Adenoviruses