pAAV-hsyn-Jaws-KGC-GFP-ER2 in Rhesus macaque (AAV5)
Addgene #65014-AAV5

Expression of Jaws on GPi  neurons. AAV5-hSyn-Jaws-KGC-GFP-ER2 was injected in the internal globus pallidus (GPi) of a rhesus macaque. The area in the rectangle is shown at higher magnification in the inset. Jaws expression was  revealed with antibodies against GFP and immunoperoxidase. GPe, external globus pallidus, OT, optic tract. ((Galvan et al., unpublished data)­

Caption: Expression of Jaws on GPi neurons. AAV5-hSyn-Jaws-KGC-GFP-ER2 was injected in the internal globus pallidus (GPi) of a rhesus macaque. The area in the rectangle is shown at higher magnification in the inset. Jaws expression was revealed with antibodies against GFP and immunoperoxidase. GPe, external globus pallidus, OT, optic tract. ((Galvan et al., unpublished data)­

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
3.60E+13 GC/ml
Injected Volume
18 μl
Injection Rate
0. 1 μl/min, increasing to 0. 2 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Globus pallidus external
Injection Site Comments
Trying to target all neurons in GPe
View in Addgene Catalog

Subject

Species / Strain
Rhesus macaque
Age at Injection
5 years (subject 1); and 5.5 years (subject 2)

Data Collection

Time After Injection
6 to 20 weeks
Detection Method
Immunohistochemistry, Optogenetics
Expression Site
Neurons at injection site (GPe neurons), neurons in neighboring regions (GPi, likely due to spread of virus), and expression of GFP along axons and terminals originating form GPe cells (anterograde transport)
Genetic Tool Outcome
The opsin Jaws silenced neurons in GPe when activated by light
Tips & Comments
Inject a smaller volume to keep the expression confined to the region of interest