AAV-hSyn1-GCaMP6s-P2A-nls-dTomato in Human (ex: human brain slices) (AAV1)
Addgene #51084-AAV1

Green channel is cells expressing GCamp

Caption: Green channel is cells expressing GCamp

Image attribution: Srinidhi Rao

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
1μl
Injection Site
Infected cells in vitro
Brain Region
Neurons cultured in a dish
Injection Site Comments
Neurons
View in Addgene Catalog

Subject

Species / Strain
Human (ex: human brain slices)
Age at Injection
10 week old neurons

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Neurons
Genetic Tool Outcome
Yes, we use to check innate calcium signals
Other Information
A small level of cell death. But very small
Tips & Comments
1uL of virus for every 24 well provides sufficient infection