pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV1)
Addgene #26969-AAV1

confocal imaging with Leica TCS SP8 VIS/405. Fluorescence in the ventral hippocampus (the virus partially spread to nearby cortex).

Caption: confocal imaging with Leica TCS SP8 VIS/405. Fluorescence in the ventral hippocampus (the virus partially spread to nearby cortex).

Image attribution: Jun Liu

confocal image taken with Leica TCS SP8 VIS/405. Fluorescenced in amygdala (projection site). The track of an optic fiber implantation into the basolateral amygdala is visible.

Caption: confocal image taken with Leica TCS SP8 VIS/405. Fluorescenced in amygdala (projection site). The track of an optic fiber implantation into the basolateral amygdala is visible.

Image attribution: Jun Liu

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.00E+13 GC/ml
Injected Volume
200 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
AAV was injected mainly into the ventral hippocampal CA1
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
10-13 weeks

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence
Expression Site
Ventral hippocampus and nearby cortex
Genetic Tool Outcome
Yes, in vivo recording in the basolateral amygdala confirmed that the ChR2 worked as expected
Tips & Comments
Inject less volume, such as 100 nl