pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)
Addgene #50477-AAV8

Image obtained on standard Nikon Fluorescent microscope, 10x objective, Texas Red channel/filter cube

Caption: Image obtained on standard Nikon Fluorescent microscope, 10x objective, Texas Red channel/filter cube

Image attribution: David Cinalli

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.60E+13 GC/ml
Injected Volume
two 0.5 μl injections
Injection Rate
0. 167 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
CA1 region of dorsal hippocampus / pyramidal neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
9-12 weeks

Data Collection

Time After Injection
6-8 weeks
Detection Method
Direct fluorescence
Expression Site
CA1 region of dorsal hippocampus / pyramidal neurons
Genetic Tool Outcome
Tool operated as expected. To activate receptors, we used DREADD Agonist 21 Dihydrochloride and CNO