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pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)

Addgene #50477-AAV8

Data Submitted By: Robert Stackman and Robert W. Stackman
Submission Date: June 26, 2019
Publication Date: June 26, 2019 (modified May 16, 2023)
Abstract: Not Provided

Image obtained on standard Nikon Fluorescent microscope, 10x objective, Texas Red channel/filter cube — Caption: Image obtained on standard Nikon Fluorescent microscope, 10x objective, Texas Red channel/filter cube
Image attribution: David Cinalli

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-CaMKIIa-hM4D(Gi)-mCherry
Addgene #50477-AAV8

Virus & Injection

Virus

Virus Name: pAAV-CaMKIIa-hM4D(Gi)-mCherry
Serotype: AAV8
Source: Addgene
Catalog Number: 50477-AAV8
Promoter: CaMKII
Cargo Type: Chemogenetics
Injection Titer / Dose: 1.60E+13 GC/mL
Injection Volume: two 0.5 μL injections
Injection Rate: 167 nL/min
Injector Material: Not Provided
Injection Site / Route: CA1 field, NeuroNames ID: 185

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Jackson Labs
Stock Number: JAX: 000664
Age at Injection: 9-12 weeks
Time After Injection: 6-8 weeks
Detection Method: Direct fluorescence
Assay & Results: CA1 region of dorsal hippocampus/pyramidal neurons.
Functional Testing: Tool operated as expected. To activate receptors, we used DREADD Agonist 21-Dihydrochloride and CNO.