pAAV-CAG-tdTomato (codon diversified) in Mouse (PHP.eB)
Addgene #59462-PHPeB

tdTomato in green; NeuroTrace 640 in magenta. Imaged on Olympus Vs120 slide scanner with 10x objective, 60 ms exposure

Caption: tdTomato in green; NeuroTrace 640 in magenta. Imaged on Olympus Vs120 slide scanner with 10x objective, 60 ms exposure

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.00E+12 GC/ml
Injected Volume
100 μl
Injection Rate
Via 0. 3 mL insulin syringe
Injection Site
Intravenous (retro-orbital or tail vein)
Injection Site Comments
Retro-orbital injection to target cells throughout the brain with the PHP. EB serotype
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
8 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Widespread infection throughout the brain. In both excitatory pyramidal neurons, as well as glia. Density was fairly uniform across cortical regions
Other Information
I didn't quantify it, but the percentage of glia infected varied across mice. I didn't see any signs of toxicity (e. G. No signs of blebbing in axons), but I also only had tdTomato
Tips & Comments
PHP. EB + CAG seems like a great combination for transducing a large numbers of neurons across the brain