pAAV-hSyn-dF-HA-KORD-IRES-mCitrine in Rat (AAV8)
Addgene #65417-AAV8

anti-mCherry (DIO-hM4D(Gi) tag) in red, anti-GFP (dF-KORD tag) in green, and anti-TH in blue brightfield/fluorescent microscope, 20x objective

Caption: anti-mCherry (DIO-hM4D(Gi) tag) in red, anti-GFP (dF-KORD tag) in green, and anti-TH in blue brightfield/fluorescent microscope, 20x objective

Image attribution: Erik Castillo

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
7.00E+12 GC/ml
Injected Volume
600 nl
Injection Rate
600 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum
Injection Site Comments
Injection site targeting dopamine neurons in Ventral Tegmental Area
View in Addgene Catalog
Secondary Virus
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
600 nl
Injection Rate
600 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum
Injection Site Comments
Targeting dopamine neurons in Ventral Tegmental Area
View in Addgene Catalog

Subject

Species / Strain
Rat Long Evans THcre
Stock Number
659 from RRRC
Age at Injection
postnatal day 60-90

Data Collection

Time After Injection
3 weeks
Detection Method
Immunofluorescence for mCherry, GFP, and TH
Expression Site
VTA, dopamine neurons
Other Information
No toxicity or inflammation seen
Tips & Comments
Since injection was at the same site, both viruses were mixed 1:1 ratio and then a total of 1. 2 ul was injected to give 600 nl of each virus. We waited 5 min before removing the injection pipette from the injection site