pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Rat (AAV2)
Addgene #44361-AAV2

endogenous fluorescence of mCherry tag, bright field/fluorescent microscope, 5x objective

Caption: endogenous fluorescence of mCherry tag, bright field/fluorescent microscope, 5x objective

Image attribution: Erik Castillo

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
750 nl
Injection Rate
500 nl/min
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum
Injection Site Comments
Injection was targeting dopamine neurons in ventral tegmental area
View in Addgene Catalog

Subject

Species / Strain
Rat Long Evans THcre
Stock Number
659 from RRRC
Age at Injection
postnatal day 60-90

Data Collection

Time After Injection
3 weeks
Detection Method
Immunofluorescence for mCherry and TH
Expression Site
VTA, dopamine neurons
Other Information
No toxicity or inflammation seen
Tips & Comments
We waited 5 min before removing the injection pipette from the injection site