pAAV-hSyn-DIO-mCherry in Mouse (AAV2)
Addgene #50459-AAV2
- Data Submitted By
- Michael Chiang and Sarah E. Ross
- Submission Date
- June 25, 2019
- Publication Date
- June 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: null
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-hSyn-DIO-mCherry
Addgene #50459-AAV2
Virus & Injection
Virus
- Virus Name
- pAAV-hSyn-DIO-mCherry
- Serotype
- AAV2
- Source
- Addgene
- Catalog Number
- 50459-AAV2
- Promoter
- Synapsin
- Cargo Type
- Control
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 500 nl
- Injection Rate
- 100 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Parabrachial nucleus, NeuroNames ID: 1927
- Additional Details
- Gad2-cre, inhibitory population.
Other Details
- Species
- Mouse
- Strain or Cell Line
- Gad2-IRES-cre on C57BL/6J background
- Source
- Jackson Labs
- Stock Number
- 10802
- Age at Injection
- 8 weeks
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- The fluorophore was expressed within a subpopulation of lateral parabrachial nucleus which need to be further characterized using FISH (experiments ongoing).
- Other Information
- AAV2 does not seem to cause neuronal blebbing as depicted here: doi: 10. 3389/fncir. 2013. 00008. AAV9 seems to be neurotoxic.
- Tips & Comments
- EF1a, CMV, CAG promoters are very strong but seem to result in fewer number of neurons infected compared to AAV under hsyn promoter.