pAAV-CaMKIIa-eNpHR 3.0-EYFP in Mouse (AAV1)
Addgene #26971-AAV1

Taken with Leica DM 5500B. Fluorescence in the dorsal hippocampus (injection site) and granular retrospenial cortex (RSC) layer 2/3 (projection site). The track of an optic fiber implantation into the dorsal RSC is visible.

Caption: Taken with Leica DM 5500B. Fluorescence in the dorsal hippocampus (injection site) and granular retrospenial cortex (RSC) layer 2/3 (projection site). The track of an optic fiber implantation into the dorsal RSC is visible.

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.00E+13 GC/ml
Injected Volume
200 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Targeting CA1 pyramidal neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
10-13 weeks

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence, in vivo electrophysiology
Expression Site
CA1, subiculum, dentate gyrus
Genetic Tool Outcome
Yes, laser stimulation activates infected neurons
Tips & Comments
Inject less volume, such as 100 nl