pAAV-Ef1a-DIO EYFP in Mouse (AAV9)
Addgene #27056-AAV9

EYFP-green  Cholinergic neurons were visualized using anti-ChAT antibody (Red)  DAPI-blue

Caption: EYFP-green Cholinergic neurons were visualized using anti-ChAT antibody (Red) DAPI-blue

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
3.30E+13 GC/ml
Injected Volume
600-800 nl
Injection Rate
200 nl for 100 nl and then 50 nl/min for 500~700 nl
Injection Site
Brain parenchyma
Brain Region
Basal forebrain
Injection Site Comments
Basal forebrain
View in Addgene Catalog

Subject

Species / Strain
Mouse B6J.129S6-Chat/MwarJ, Homozygous for Chat
Stock Number
28861
Age at Injection
8-12 weeks

Data Collection

Time After Injection
6 weeks
Detection Method
Immunohistochemistry
Expression Site
Basal forebrain Cholinergic neurons and non-cholinergic neurons (less than 2%)
Genetic Tool Outcome
The blue laser was used (Control group)