pAAV-hSyn-hM4D(Gi)-mCherry in Rat (AAV Retrograde)
Addgene #50475-AAVrg

Zeiss LSM 710 Confocal, 10x in prelimbic   568 channel for DsRed staining (Living Colors rabbit polyclonal antibody)

Caption: Zeiss LSM 710 Confocal, 10x in prelimbic 568 channel for DsRed staining (Living Colors rabbit polyclonal antibody)

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
two 750 nl injections
Injection Rate
150 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Pyramidal tract cortical neurons
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague Dawley
Age at Injection
~2-3 months old

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Expression Site
Pyramidal tract neurons in motor, sensory, cingulate, and prelimbic cortices. Some expression in ventroorbital cortex and infralimbic cortex as well
Genetic Tool Outcome
Yes, there was CNO-induced behavioral changes seen in animals expressing Gi DREADD versus vehicle controls
Tips & Comments
This injection can be very bloody. It may be smart to go at an angle for pons targeting