pAAV.Syn.NES-jRGECO1a.WPRE.SV40 in Rat (AAV Retrograde)
Addgene #100854-AAVrg

Imaged in 568 channel on a Zeiss LSM 710 confocal at 10x

Caption: Imaged in 568 channel on a Zeiss LSM 710 confocal at 10x

488 channel for GCaMP expression imaging on a Zeiss LSM 710 confocal 10x

Caption: 488 channel for GCaMP expression imaging on a Zeiss LSM 710 confocal 10x

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
1000 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Targeting intratelencephalic (IT) cortical neurons projecting to the nucleus accumbens
View in Addgene Catalog
Secondary Virus
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)
Promoter
Syn
Injected Titer
9.00E+12 GC/ml
Injected Volume
1000 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Pyramidal tract (PT) cortical projection neurons projecting to the pons. Injected at same session as jRGECO
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague Dawley
Age at Injection
~2-3 months old

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry, fiber photometry recordings
Expression Site
GCaMP PT expression, jRGECO IT expression
Genetic Tool Outcome
We have had issues with obtaining functional recordings during fiber photometry. Have had issues with jRGECO not expressing in some cohorts
Tips & Comments
Maybe try to dilute the virus (particuarly GCaMP) and inject a lower volume