pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV5)
Addgene #50477-AAV5

Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
9.50E+12 GC/ml
Injected Volume
80 nl
Injection Rate
0. 08 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Thalamus
Injection Site Comments
Virus was targeted to the thalamic nucleus reuniens
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
9-12 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Putative CaMKII+ cells in nucleus reuniens (RE) were labeled
Genetic Tool Outcome
CNO was used to activate the receptor
Tips & Comments
Virus expression was very strong, I would have liked to perform a range of dilutions