pAAV-CaMKIIa-hM3D(Gq)-mCherry in Rat (AAV5)
Addgene #50476-AAV5

Red = hM3Dq-mCherry expression  Green = Fos protein (Alexa Fluor 488)

Caption: Red = hM3Dq-mCherry expression Green = Fos protein (Alexa Fluor 488)

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
3.10E+13 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Thalamus
Injection Site Comments
CaMKIIa-positive cells in Medial Dorsal Thalamus
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague-Dawley, male
Age at Injection
9-11 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
CaMKIIa-positive cells in medial dorsal thalamus
Genetic Tool Outcome
Yes, CNO (3 mg/kg; i. P. ) induced Fos protein 90 minutes post injection