pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 in Mouse (AAV9)
Addgene #100834-AAV9

Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Image attribution: Kyle Puhger

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
2.50E+13 GC/ml
Injected Volume
250 nl
Injection Rate
0. 16 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
CA1 subregion of dorsal hippocampus
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
9-12 weeks

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Putative CaMKII+ pyramidal cells in CA1 subregion
Genetic Tool Outcome
GCaMP signals in dorsal CA1 were collected via fiber photometry