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pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)

Addgene #44362-AAV8

Data Submitted By
Michael Baratta
Submission Date
June 20, 2019
Publication Date
June 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: DOI:http://dx.doi.org/10.1523/ENEURO.0025-18.2018 Figure 5. "Available for the public to copy, distribute, or display under the Creative Commons Attribution 4.0 International (CC BY 4.0) license." https://creativecommons.org/licenses/by/4.0/
Image attribution: null

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Addgene #44362-AAV8

Virus & Injection

Virus

Virus Name
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
44362-AAV8
Promoter
Synapsin
Cargo Type
Chemogenetics
Injection Titer / Dose
1.90E+13 GC/mL
Injection Volume
1μL
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Sprague-Dawley, female
Source
Not provided
Age at Injection
9-11 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Prelimbic neurons that project to the dorsal raphe nucleus.
Target Cell Transduction
Yes, expression was restricted to expected pyramidal cell layer.
Other Information
No toxicity was detected