pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8

DOI:http://dx.doi.org/10.1523/ENEURO.0025-18.2018  Figure 5. Available for the public to copy, distribute, or display under the Creative Commons Attribution 4.0 International (CC BY 4.0) license." https://creativecommons.org/licenses/by/4.0/

Caption: DOI:http://dx.doi.org/10.1523/ENEURO.0025-18.2018 Figure 5. Available for the public to copy, distribute, or display under the Creative Commons Attribution 4.0 International (CC BY 4.0) license." https://creativecommons.org/licenses/by/4.0/

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.90E+13 GC/ml
Injected Volume
1μl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Prelimbic region of the medial prefrontal cortex; Prelimbic neurons that project to the dorsal raphe nucleus
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague-Dawley, female
Age at Injection
9-11 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Prelimbic cortex, Prelimbic neurons that project to the dorsal raphe nucleus
Genetic Tool Outcome
Yes, expression was restricted to expected pyramidal cell layer
Other Information
No toxicity was detected