pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9

Cholinergic neurons were visualized using anti-ChAT+ antibody (Red). Virus(ChR2-EYFP) expressing neurons were green.

Caption: Cholinergic neurons were visualized using anti-ChAT+ antibody (Red). Virus(ChR2-EYFP) expressing neurons were green.

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
2.20E+13 GC/ml
Injected Volume
600-800 nl
Injection Rate
200 nl/min for 100 nl and 50 nl/min for 500~700 nl
Injection Site
Brain parenchyma
Brain Region
Basal forebrain
Injection Site Comments
Cholinergic neurons in the basal forebrain
View in Addgene Catalog

Subject

Species / Strain
Mouse B6J.129S6-Chat/MwarJ, Genotype: Homozygous for Chat #028861 from Jackson Labs
Stock Number
28861
Age at Injection
8-12 weeks

Data Collection

Time After Injection
6 weeks
Detection Method
Immunohistochemistry
Expression Site
Basal forebrain region. ChAT+ cholinergic neurons and non-cholienrgic neurons (less than 2% among virus expressed cells)
Genetic Tool Outcome
The blue laser was used to activate the terminal of the cholinergic neurons which express the ChR2
Tips & Comments
Wait ~20min after injection