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pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)

Addgene #20298-AAV9

Data Submitted By
Gaqi Tu and Kaori Takehara-Nishiuchi
Submission Date
June 23, 2019
Publication Date
June 23, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Cholinergic neurons were visualized using anti-ChAT+ antibody (Red). Virus(ChR2-EYFP) expressing neurons were green.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Addgene #20298-AAV9

Virus & Injection

Virus

Virus Name
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Serotype
AAV9
Source
Addgene
Catalog Number
20298-AAV9
Promoter
EF1a
Cargo Type
Optogenetics
Injection Titer / Dose
2.20E+13 GC/mL
Injection Volume
600-800 nL
Injection Rate
200 nL/min for 100 nL and 50 nL/min for 500~700 nL
Injector Material
Not Provided
Injection Site / Route
Basal forebrain, NeuroNames ID: 1997
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6J.129S6-Chat/MwarJ
Source
Jackson Labs
Stock Number
28861
Other Information
Genotype: Homozygous for Chat.
Age at Injection
8-12 weeks
Time After Injection
6 weeks
Detection Method
Immunohistochemistry
Assay & Results
Basal forebrain region. ChAT+ cholinergic neurons and non-cholienrgic neurons (less than 2% among virus expressed cells).
Functional Testing
The blue laser was used to activate the terminal of the cholinergic neurons which express the ChR2.
Tips & Comments
Wait ~20min after injection.