pAAV-hSyn-DIO-mCherry in Rat (AAV8)
Addgene #50459-AAV8

Prelimbic neurons that project to dorsal raphe nucleus, Zeiss 510 Confocal Laser Scanning Microscope, 543 nm laser, 20X objective

Caption: Prelimbic neurons that project to dorsal raphe nucleus, Zeiss 510 Confocal Laser Scanning Microscope, 543 nm laser, 20X objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.10E+13 GC/ml
Injected Volume
1 μl per hemisphere
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Prelimbic region of the medial prefrontal cortex; Prelimbic neurons that project to the dorsal raphe nucleus
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague Dawley
Age at Injection
9-11 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Prelimbic region of the medial prefrontal cortex; Prelimibic neurons that project to the dorsal raphe nucleus
Genetic Tool Outcome
Yes, mCherry expression was restricted to dorsal raphe-projecting prelimbic neurons (Cre-positive)
Other Information
No toxicity was observed, minimal damage surrounding injection site
Tips & Comments
Expression was robust