pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg

Tdtomato flourescence observed in a 300 um coronal brain slice on an upright Olympus BX53-series microscope.

Caption: Tdtomato flourescence observed in a 300 um coronal brain slice on an upright Olympus BX53-series microscope.

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.00E+13 GC/ml
Injected Volume
200 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Amygdala
Injection Site Comments
Aiming to target pyramidal cells in the basolateral amygdala. We also targeted medium spiny neurons in the anterior nucleus accumbens, at the interface between the core and shell. The aim was to record from pyramidal cells in deep layer prelimbic prefrontal cortex, which we found with extensive tdTomato expression after this procedure
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
4-5 weeks

Data Collection

Time After Injection
1 week
Detection Method
Direct fluorescence
Expression Site
Neurons within the injection site as well as pyramidal cells throughout the prefrontal cortex
Genetic Tool Outcome
Not applicable
Other Information
Worked wonderfully
Tips & Comments
No. Very straightforward