pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV5)
Addgene #44361-AAV5

We used the Zeiss LSM 880 Confocal Microscope (Objective, 10X; Channel 1: laser, 561 nm, color, red; Channel 2: laser, 408 nm) to image the target regions. We used the DAPI (SC-3598, Santa Cruz Biotechnology) to identify the cell nucleus.

Caption: We used the Zeiss LSM 880 Confocal Microscope (Objective, 10X; Channel 1: laser, 561 nm, color, red; Channel 2: laser, 408 nm) to image the target regions. We used the DAPI (SC-3598, Santa Cruz Biotechnology) to identify the cell nucleus.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.7E12-1.2E13 GC/ml
Injected Volume
300 nl
Injection Rate
5 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
We delivered the virus into the layer 3 anterior piriform cortex (APC) of the parvalbumin (PV)-Cre transgenic mice
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Jackson Labs
Age at Injection
3 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
The expression thus was only observed in the PV interneurons of APC L3