pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8

Alex fluor 568 antibody   568 channel  Zeiss LSM 710 confocal on 10x objective

Caption: Alex fluor 568 antibody 568 channel Zeiss LSM 710 confocal on 10x objective

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.00E+13 GC/ml
Injected Volume
750 nl
Injection Rate
150 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Pyramidal tract neurons in the orbitofrontal cortex (CRE injected in the pons)
View in Addgene Catalog
Secondary Virus
CAV2-CRE
Injected Titer
GC/ml
Injected Volume
1 μl injections
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Pyramidal tract neurons. Surgeries for both CAV and AAV injections were performed the same day. The order of injections was alternated

Subject

Species / Strain
Rat Sprague Dawley; Long Evans
Age at Injection
~2-3 months

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Expression Site
Around lateral and ventral orbital cortex
Genetic Tool Outcome
CNO (5 mg/kg) was used for transient inhibition of pyramidal tract neurons. Within subject controls were utilized. Expression was successfully verified with living colors ds red rabbit polyclonal antibody