pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8

red: direct fluorescence for mCherry in the construct.  blue: DAPI

Caption: red: direct fluorescence for mCherry in the construct. blue: DAPI

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.00E+12 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
Targeting Drd1 cells in suprachiamatic nucleus (SCN)
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
10 weeks

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Targeted region (SCN) and part of PVN
Genetic Tool Outcome
Yes. 0. 3 mg/kg CNO ip. Injection produces expected behavior and c-fos expression
Tips & Comments
AAV8 does not penetrate SCN as good as AAV1