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pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)

Addgene #50477-AAV8

Data Submitted By
Brittney Cox and Christine Gall
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Dorsal CA3; mcherry staining, epi fluorescence microscope, 10x

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Addgene #50477-AAV8

Virus & Injection

Virus

Virus Name
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Serotype
AAV8
Source
Addgene
Catalog Number
50477-AAV8
Promoter
CaMKII
Cargo Type
Chemogenetics
Injection Titer / Dose
2.60E+12 GC/ml
Injection Volume
3 μl for hippocampal subregions, 8 μl for entorhial cortex regions
Injection Rate
Not Provided
Injector Material
Not Provided
Injection Site / Route
Hippocampus NeuroNames ID : 3157
Additional Details
I specifically injected CA1, CA3, and dentate gyrus. Additionally, lateral and medial entorhinal cortex; Forebrain: Hippocampus; CA1 & CA3=pyramidal cells. Dentate gyrus= granual cells. Entorhinal cortex= pyramidal cells
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
Wild type FVB129
Source
Not provided
Age at Injection
2 months
Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Assay & Results
Not Provided
Functional Testing
CNO was used at 1 mg/kg systemically and 10 uM in slice electrophysiology
Tips & Comments
Previously tried serotype 5 for these experiments and we got some retrograde labeling, so 8 worked best