pAAV-CaMKIIa-hM4D(Gi)-mCherry in Mouse (AAV8)
Addgene #50477-AAV8

Dorsal CA3;  mcherry staining, epi fluorescence microscope, 10x

Caption: Dorsal CA3; mcherry staining, epi fluorescence microscope, 10x

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
2.60E+12 GC/ml
Injected Volume
3 μl for hippocampal subregions, 8 μl for entorhial cortex regions
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
I specifically injected CA1, CA3, and dentate gyrus. Additionally, lateral and medial entorhinal cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse Wild type FVB129
Age at Injection
2 months

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
CA1 & CA3=pyramidal cells. Dentate gyrus= granual cells. Entorhinal cortex= pyramidal cells
Genetic Tool Outcome
CNO was used at 1 mg/kg systemically and 10 uM in slice electrophysiology
Tips & Comments
Previously tried serotype 5 for these experiments and we got some retrograde labeling, so 8 worked best