pAAV-hSyn-dLight1.2 in Mouse (AAV5)
Addgene #111068-AAV5

Inscopix one-photon miniscope

Caption: Inscopix one-photon miniscope

Image attribution: Henry Hallock

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
8.30E+12 GC/ml
Injected Volume
two 400 nl injections
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Targeted prelimbic sub-region of medial prefrontal cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
8 weeks
Detection Method
in vivo endoscopic imaging
Expression Site
Prelimbic neurons
Genetic Tool Outcome
Tool did work - amphetamine (3 mg/kg) was used to increase dopamine levels in cortex
Other Information
No toxicity - imaged with an Inscopix miniature head-mounted microscope and an endoscopic lens
Tips & Comments
Fluorescence was low with one-photon imaging, and couldn't make out individual ROIs, but can be used for bulk imaging