pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV2)
Addgene #44362-AAV2

anti-mCherry in red, anti-substance P in green, bright field/fluorescent microscope, 5x objective

Caption: anti-mCherry in red, anti-substance P in green, bright field/fluorescent microscope, 5x objective

Image attribution: Erik Castillo and Mitchell Farrell

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
300 nl
Injection Rate
300 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Globus pallidus external
Injection Site Comments
Targetting GABA neurons in Ventral Pallidum
View in Addgene Catalog

Subject

Species / Strain
Rat GAD-iCre obtained from RRRC
Stock Number
751 from RRRC
Age at Injection
postnatal day 60-90

Data Collection

Time After Injection
3 weeks
Detection Method
Immunofluorescence staining for mCherry and Substance P
Expression Site
Ventral Pallidum, GABA neurons
Other Information
No toxicity or inflammation was seen
Tips & Comments
This virus had dorsal spread in GADiCre transgenic rats if the pipette was not removed very slowly after injection. We also waited 7 min before removing the pipette