pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV9)
Addgene #26969-AAV9

Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Image attribution: Kyle Puhger

Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Caption: Images were acquired at 20× magnification using a fluorescence slide scanner (BX61VS, Olympus).

Image attribution: Kyle Puhger

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.50E+13 GC/ml
Injected Volume
250 nl
Injection Rate
0. 12 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Viral injections were targeted to the dorsal CA1 subregion of the hippocampus
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
10-14 weeks

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Expression Site
Putative CaMKII+ cells in the pyramidal layer of the CA1 subregion
Genetic Tool Outcome
473nm blue light was used to activate the ChR2 opsin