pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg

20x on an LSM 700 with 555 (tdTomato), 647 (Fos), and 405 (DAPI) nm wavelengths. The brain region is the ventral hippocampus, and the dotted line is the boundary of the CA1 subfield.

Caption: 20x on an LSM 700 with 555 (tdTomato), 647 (Fos), and 405 (DAPI) nm wavelengths. The brain region is the ventral hippocampus, and the dotted line is the boundary of the CA1 subfield.

Image attribution: Henry Hallock

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.10E+13 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injected into prelimbic sub-region of medial prefrontal cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Genetic Tool Outcome
Used standard confocal microscopy to detect tdTomato protein, as well as tdTomato mRNA following single-molecule in situ hybridization
Other Information
TdTomato protein expressed well in ventral hippocampus, locus coeruleus, and mediodorsal thalamus projections to prelimbic cortex, less so in basal forebrain and ventral tegmental area
Tips & Comments
See above - tropism of AAVrg seems to be limited to certain cell types/regions, but expresses well in cortex, hippocampus, locus coeruleus