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pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)

Addgene #59462-AAVrg

Data Submitted By
Henry Hallock and Keri Martinowich
Submission Date
June 19, 2019
Publication Date
June 19, 2019 (modified May 16, 2023)
Abstract
Not Provided
20x on an LSM 700 with 555 (tdTomato), 647 (Fos), and 405 (DAPI) nm wavelengths. The brain region is the ventral hippocampus, and the dotted line is the boundary of the CA1 subfield.
Caption: 20x on an LSM 700 with 555 (tdTomato), 647 (Fos), and 405 (DAPI) nm wavelengths. The brain region is the ventral hippocampus, and the dotted line is the boundary of the CA1 subfield.
Image attribution: Henry Hallock

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-CAG-tdTomato (codon diversified)
Addgene #59462-AAVrg

Virus & Injection

Virus

Virus Name
pAAV-CAG-tdTomato (codon diversified)
Serotype
AAV Retrograde
Source
Addgene
Catalog Number
59462-AAVrg
Promoter
CAG
Cargo Type
Control
Injection Titer / Dose
1.10E+13 GC/mL
Injection Volume
600 nL
Injection Rate
200 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
Additional Details
Injected into prelimbic sub-region of medial prefrontal cortex.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
JAX: 000664
Age at Injection
3 months
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Used standard confocal microscopy to detect tdTomato protein, as well as tdTomato mRNA following single-molecule in situ hybridization. TdTomato protein expressed well in ventral hippocampus, locus coeruleus, and mediodorsal thalamus projections to prelimbic cortex, less so in basal forebrain and ventral tegmental area.
Target Cell Transduction
See above - tropism of AAVrg seems to be limited to certain cell types/regions, but expresses well in cortex, hippocampus, locus coeruleus.