pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV2)
Addgene #50474-AAV2

10x image of expressing cell bodies in medial septum. Red is endogeous mCherry reporter. Green is mouse mCherry primary (1:8000, Abcam-ab125096) and goat anti-mouse secondary stain (alexa 488, 1:500; Abcam-ab150113). Yellow is co-stain overlay. Blue is Dapi.

Caption: 10x image of expressing cell bodies in medial septum. Red is endogeous mCherry reporter. Green is mouse mCherry primary (1:8000, Abcam-ab125096) and goat anti-mouse secondary stain (alexa 488, 1:500; Abcam-ab150113). Yellow is co-stain overlay. Blue is Dapi.

Image attribution: David Bortz

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
3.00E+12 GC/ml
Injected Volume
8 μL- 1 injection
Injection Rate
0. 1 μl/min
Injection Site
Brain parenchyma
Brain Region
Medial septum
Injection Site Comments
I was trying to hit the anterior-posterior extent of the medial septum without getting into lateral septum or other parts of the basal forebrain
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague Dawley, male from Envigo
Age at Injection
Young adult (weight 300-325 grams)

Data Collection

Time After Injection
8 weeks for cell body expression, 12 weeks for terminal expression.
Detection Method
Immunohistochemistry
Expression Site
Good expression on cell bodies within the medial septum as well as terminals in terminating regions. Cell type unknown
Other Information
No toxicity detected
Tips & Comments
The one tip I would offer would be to leave the syringe at the place of infusion for several minutes following the infusion, and then raise it very slowly. This procedure has been effective for us in minimizing "dragging" the virus up with the syringe and out of the region of interest