pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 in Mouse (AAV1)
Addgene #100853-AAV1

In vivo two-photon imaging at 1020 nm for excitation and ~610 nm for emission. PV-expressing GABAergic interneurons in the mouse neocortex, specifically dorsal anterior cingulate cortex/medial secondary motor cortex.

Caption: In vivo two-photon imaging at 1020 nm for excitation and ~610 nm for emission. PV-expressing GABAergic interneurons in the mouse neocortex, specifically dorsal anterior cingulate cortex/medial secondary motor cortex.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
180 nl
Injection Rate
40 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse, superficial layers Cell type: parvalbumin-expressing GABAergic neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse cross between 5xFAD (JAX Stock Number 006554) and PV-Cre animal (JAX 008069), with Cre expressed in PV neurons.
Age at Injection
8-24 weeks

Data Collection

Time After Injection
5 weeks
Detection Method
Direct fluorescence
Expression Site
Parvalbumin-expressing GABAergic neurons