pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV2)
Addgene #44361-AAV2

Green: immuno-staining with an antibody against melanopsin; Red: fluorescence signal from hM3D-mCherry.

Caption: Green: immuno-staining with an antibody against melanopsin; Red: fluorescence signal from hM3D-mCherry.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
2 μl per eye
Injection Site
Retina
Injection Site Comments
Targeting retinal ganglion cells
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J background (Opn4-Cre is a BAC-transgene that we inserted into the mouse genome so that Cre recombinase is expressed in Opn4-positive cells)
Age at Injection
1-3 months

Data Collection

Time After Injection
8 weeks
Detection Method
Direct fluorescence
Expression Site
Virus were injected into Opn4-Cre mice. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells ended up expressing hM3D from viral infection
Genetic Tool Outcome
Yes