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pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV5)

Addgene #44361-AAV5

Data Submitted By: Huanhuan Li and Geoffrey Lau
Submission Date: June 20, 2019
Publication Date: June 20, 2019 (modified May 16, 2023)
Abstract: Not Provided

We used the Zeiss LSM 880 Confocal Microscope (Objective, 10X; Channel 1: laser, 561 nm, color, red; Channel 2: laser, 408 nm) to image the target regions. We used the DAPI (SC-3598, Santa Cruz Biotechnology) to identify the cell nucleus. — Caption: We used the Zeiss LSM 880 Confocal Microscope (Objective, 10X; Channel 1: laser, 561 nm, color, red; Channel 2: laser, 408 nm) to image the target regions. We used the DAPI (SC-3598, Santa Cruz Biotechnology) to identify the cell nucleus.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Addgene #44361-AAV5

Virus & Injection

Virus

Virus Name: pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Serotype: AAV5
Source: Addgene
Catalog Number: 44361-AAV5
Promoter: Synapsin
Cargo Type: Chemogenetics
Injection Titer / Dose: 4.7E+12-1.2E+13 GC/mL
Injection Volume: 300 nL
Injection Rate: 300 nL/min
Injector Material: Not Provided
Injection Site / Route: Piriform area, NeuroNames ID: 165

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Other Details

Species: Mouse
Strain or Cell Line: C57/BL6J
Source: Jackson Labs
Age at Injection: 3 weeks
Time After Injection: 3 weeks
Detection Method: Immunohistochemistry
Assay & Results: We delivered the virus into the layer 3 anterior piriform cortex (APC) of the parvalbumin (PV)-Cre transgenic mice. The expression thus was only observed in the PV interneurons of APC L3.