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pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA in Mouse (AAV1)

Addgene #20297-AAV1

Data Submitted By: Sindhuja Baskar and David Margolis
Submission Date: October 25, 2019
Publication Date: October 25, 2019 (modified May 16, 2023)
Abstract: Not Provided

Confocal microscope image of striatal cell projections from posterior medial nucleus of the thalamus (POm) — Caption: Confocal microscope image of striatal cell projections from posterior medial nucleus of the thalamus (POm)
Image attribution: Alex Yonk

Any further use of this image requires permission from the lab who submitted the data.

Vector Used: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Addgene #20297-AAV1

Virus & Injection

Virus

Virus Name: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Serotype: AAV1
Source: Addgene
Catalog Number: 20297-AAV1
Promoter: EF1a
Cargo Type: Optogenetics
Injection Titer / Dose: 8.78E+12 GC/mL
Injection Volume: ~280 nL
Injection Rate: 28 nL/min
Injector Material: Not Provided
Injection Site / Route: Posteromedian thalamic nucleus, NeuroNames ID: 1106
Additional Details: One injection over the course of 8-10 minutes.

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: B6; Tg(Gpr26-cre)KO250
Stock Number: RRID:MMRRC_036915-UCD
Age at Injection: 39-40 days
Time After Injection: 3 weeks
Detection Method: Direct fluorescence