Anti-GluA2 [L21/32R] in Western Blot (Mouse)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-GluA2 [L21/32R] (hybridoma-derived) in a western blot assay with a mouse sample.

Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with L21/32 (left) or N52A/42 (right) TC supe. — Caption: Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with L21/32 (left) or N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Immunoblot of membranes from adult rat brain (RBM) and adult GluA2/GluR2 knockout (KO) and wild-type (WT) mouse hippocampi (MHM). Mouse samples courtesy of Richard Huganir (Johns Hopkins University). — Caption: Immunoblot of membranes from adult rat brain (RBM) and adult GluA2/GluR2 knockout (KO) and wild-type (WT) mouse hippocampi (MHM). Mouse samples courtesy of Richard Huganir (Johns Hopkins University).
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-GluA2 [L21/32R]
Addgene #184172

Materials & Methods

Sample

Species: Mouse
Cell / Tissue Type: Brain, hippocampus

Primary Antibody

Name: Anti-GluA2 [L21/32R]
Species: Mouse
Gene / Target: Gria2

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes