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Anti-Giantin [TA10] - Chimeric in Immunocytochemistry (Human)

Addgene #199193

Data Submitted By: Kate Harten
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 29, 2024)
Abstract: Experimental results for Anti-Giantin [TA10] - Chimeric (Addgene Antibody 199193) in an immunocytochemistry assay with a human sample.

HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh571). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh571). — Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh571). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh571).
Image attribution: Addgene

HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh752). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh752). — Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh752). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh752).
Image attribution: Addgene

HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh837). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh837). — Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh837). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh837).
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-Giantin [TA10] - Chimeric
Addgene #199193; Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Thermo Fisher #A-11012

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: HeLa

Buffer

Name: PBS

Fixative

Fixative: Formaldehyde/Paraformaldehyde

Permeabilization

Agent: 0.2% Triton X-100 in PBS
Time: 10 min
Temperature: Room Temperature

Blocking

Agent: BSA
Concentration: 5%
Time: 20 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-Giantin [TA10] - Chimeric
Source: Addgene
Catalog Number: 199193
Species: Rabbit
Gene / Target: GOLGB1
Concentration: 0.1 µg/mL
Time: 90 min
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Source: Thermo Fisher
Catalog Number: A-11012
Concentration: 2 µg/mL
Time: 30 min
Temperature: Room Temperature

Additional Information

Additional Information: HeLa cells were untreated or transduced with shRNA. Stable cells were selected and stained with Anti-Giantin [TA10]
Protocol: Addgene immunocytochemistry protocol

Results

Result: Pass (The antibody worked under these conditions)