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Anti-Giantin [TA10] - Chimeric in Immunocytochemistry (Human)

Addgene #199193

Data Submitted By
Kate Harten
Lab Name
Addgene
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified March 29, 2024)
Abstract
Experimental results for Anti-Giantin [TA10] - Chimeric (Addgene Antibody 199193) in an immunocytochemistry assay with a human sample.
Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh571). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh571).
Image attribution: Addgene
Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh752). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh752).
Image attribution: Addgene
Caption: HeLa cells were untreated or transduced with a scrambled shRNA (shscrambled) or an shRNA against Giantin (sh837). Stable cells were selected and stained with Anti-Giantin [TA10]. Giantin (red) is visible in the untreated and shscrambled lines but reduced in the Giantin knockdown line (sh837).
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-Giantin [TA10] - Chimeric
Addgene #199193
Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Thermo Fisher #A-11012

Materials & Methods

Sample

Species
Human
Cell / Tissue Type
HeLa

Buffer

Name
PBS

Fixative

Fixative
Formaldehyde/Paraformaldehyde

Permeabilization

Agent
0.2% Triton X-100 in PBS
Time
10 min
Temperature
Room Temperature

Blocking

Agent
BSA
Concentration
5%
Time
20 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-Giantin [TA10] - Chimeric
Source
Addgene
Catalog Number
199193
Species
Rabbit
Gene / Target
GOLGB1
Concentration
0.1 µg/mL
Time
90 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Source
Thermo Fisher
Catalog Number
A-11012
Concentration
2 µg/mL
Time
30 min
Temperature
Room Temperature

Additional Information

Additional Information

HeLa cells were untreated or transduced with shRNA. Stable cells were selected and stained with Anti-Giantin [TA10]

Protocol
Addgene immunocytochemistry protocol

Results

Result
Pass (The antibody worked under these conditions)