Anti-Mortalin/GRP75 [N52A/42.1R] in Western Blot (Human)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-Mortalin/GRP75 [N52A/42.1R] (hybridoma-derived) in a western blot assay with a human sample.

Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe. — Caption: Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

Immunoblot against HEK293 cells transfected as shown. Note that GRP75 is endogenously expressed in HEK (and COS) cells and the relatively weak RBM staining is due to short film exposure time. Cell samples courtesy of Gino Cortopassi (UC Davis). — Caption: Immunoblot against HEK293 cells transfected as shown. Note that GRP75 is endogenously expressed in HEK (and COS) cells and the relatively weak RBM staining is due to short film exposure time. Cell samples courtesy of Gino Cortopassi (UC Davis).

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-Mortalin/GRP75 [N52A/42.1R]
Addgene #180091

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: Cerebral cortex, cerebellum, hippocampus, HEK293 cells

Primary Antibody

Name: Anti-Mortalin/GRP75 [N52A/42.1R]
Species: Mouse
Gene / Target: Hspa9

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)