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Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] in Immunohistochemistry (Rat)

Addgene #190892

Data Submitted By: James Trimmer
Lab Name: James Trimmer, UC Davis
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] (Addgene Antibody 190892) in an immunohistochemistry assay with a rat sample.

Free-floating rat brain sections were fixed and stained with purified Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] hybridoma-derived tissue culture supernatant. The staining patterns from antibody produced at Addgene (K9/40R) were compared to UC Davis-derived antibody (K9/40 TC Supernatant). — Caption: Free-floating rat brain sections were fixed and stained with purified Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] hybridoma-derived tissue culture supernatant. The staining patterns from antibody produced at Addgene (K9/40R) were compared to UC Davis-derived antibody (K9/40 TC Supernatant).
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R]
Addgene #190892; mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #115-065-205, 115-065-206, 115-065-207

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Fixative

Fixative: Formaldehyde/Paraformaldehyde

Permeabilization / Delipidation

Agent: PBS containing 1:2000 avidin and 0.5% Triton X-100
Time: 45 min
Temperature: Room Temperature

Blocking

Agent: Serum
Concentration: 0.5%
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R]
Source: Addgene
Catalog Number: 190892
Species: Mouse
Gene / Target: Kcnab1
Concentration: 1 µg/mL
Time: overnight
Temperature: 4 °C

View in Addgene Catalog

Secondary Antibody

Name: mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Source: Jackson Labs
Catalog Number: 115-065-205, 115-065-206, 115-065-207
Concentration: 0.5 µg/mL
Time: 1 hour
Temperature: Room Temperature

Additional Information

Additional Information

Tissue type: Free-floating brain sections

Antigen-retrieval: No

Protocol

Trimmer lab immunohistochemistry protocol

Results

Result: Pass (The antibody worked under these conditions)