Anti-Kv1.6 K+ channel [K19/36R] in Immunohistochemistry (Mouse)
Addgene #184211
- Data Submitted By
- Zhuhao Wu
- Lab Name
- Zhuhao Wu, Weill Cornell Medicine
- Submission Date
- March 20, 2024
- Publication Date
- October 17, 2024 (modified November 11, 2024)
- Abstract
- Experimental results for Anti-Kv1.6 K+ channel [K19/36R] (Addgene #184211) in an immunohistochemistry assay with a mouse sample.
![Delipidated mouse brain tissue was stained with Anti-Kv1.6 K+ channel [K19/36R].](https://datahub-media.addgene.org/report-images/ynofqi/184211-Immunohistochemistry-2023-08-28_160747.014027.jpg)
Image attribution: Zhuhao Wu, Weill Cornell Medicine
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Kv1.6 K+ channel [K19/36R]
Addgene #184211 -
HRP-conjugated AffiniPure Goat Anti-Mouse IgG, Fc_ subclass 2a specific
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Species
- Mouse
- Cell / Tissue Type
- 70 µm mounted sagittal section
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Delipidation
- Yes
Blocking
- Agent
- B1n
- Time
- 3x 1 hour
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Kv1.6 K+ channel [K19/36R]
- Source
- Addgene
- Catalog Number
- 184211
- Species
- Mouse
- Gene / Target
- Kcna6
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- Room Temperature
Secondary Antibody
- Name
- HRP-conjugated AffiniPure Goat Anti-Mouse IgG, Fc_ subclass 2a specific
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1 µg/mL
Additional Information
- Additional Information
Tissue type: 70 µm mounted sagittal section
Antigen-retrieval: No
The staining pattern was compared to the RNA expression profile from the Allen Brain Atlas In Situ Hybridization Reference Database (Link opens in a new window)
- Protocol
- Wu lab thick-section immunohistochemistry protocols (Link opens in a new window)
Results
- Result
- Inconclusive
- Describe Results
- The staining is not clear and no controls are shown, so data is inconclusive.