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Anti-FTH1 [GP5] in Western Blot (Human)

Addgene #198270

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-FTH1 [GP5] (Addgene Antibody 198270) in a western blot assay with a human sample.

Purified recombinant 6xHis-FTH1 Met1-Ser183 or 7xHis-P-RAN-RFP2 were immunoblotted against FTH1 (198270) or His (184180). — Caption: Purified recombinant 6xHis-FTH1 Met1-Ser183 or 7xHis-P-RAN-RFP2 were immunoblotted against FTH1 (198270) or His (184180).
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-FTH1 [GP5]
Addgene #198270; Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: Purified protein

Buffer

Name: TBS-T
pH: 7.4

Blocking

Agent: Milk
Concentration: 5%
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-FTH1 [GP5]
Source: Addgene
Catalog Number: 198270
Species: Mouse
Gene / Target: FTH1
Concentration: 5 µg/mL
Time: 45 min
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: Goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information: Purified recombinant 6xHis-FTH1 or 7xHis-RAN-RFP2 was reduced and separated by denaturing SDS-PAGE
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 23.4 kDa

Results

Result: Pass (The antibody worked under these conditions)