Anti-Arl13b [N295B/66R] - Chimeric in Western Blot (Rat)
Addgene #198001
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified March 29, 2024)
- Abstract
- Experimental results for Anti-Arl13b [N295B/66R] - Chimeric (Addgene Antibody 198001) in a western blot assay with a rat sample.
![Crude rat membranes immunoblotted against Anti-Arl13b [N295B/66R] recombinant mouse antibody (180085) or the recombinant chimeric rabbit antibody (198001).](https://datahub-media.addgene.org/report-images/ybwd5z/198001-Western_Blot-2023-03-30_133704.249505.png)
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Arl13b [N295B/66R] - Chimeric
Addgene #198001 -
goat anti-rabbit IgG Fc, HRP
Thermo Fisher #A16116
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Total Amount
- 30 µg
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Arl13b [N295B/66R] - Chimeric
- Source
- Addgene
- Catalog Number
- 198001
- Species
- Rabbit
- Gene / Target
- Arl13b
- Concentration
- 2 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- goat anti-rabbit IgG Fc, HRP
- Source
- Thermo Fisher
- Catalog Number
- A16116
- Species
- Rabbit
- Concentration
- unknown
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Goat anti-mouse IgG (H+L) HRP (Thermo Fisher, catalog number A16078) used to detect 180085
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 60 kDa
Results
- Result
- Pass (The antibody worked under these conditions)