Anti-Botch [N116/14R] in Immunocytochemistry
Addgene #191818
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Botch [N116/14R] (Addgene Antibody 191818) in an immunocytochemistry assay with a other sample.
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Botch [N116/14R]
Addgene #191818 -
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
Thermo Fisher #A21137
Materials & Methods
Sample
- Species
- Other
- Cell / Tissue Type
- COS
Buffer
- Name
- TBS
- pH
- 7.5
Fixative
- Fixative
- 3% Paraformaldehdye, +Ca, +Mg in PBS pH 7.4
Permeabilization
- Agent
- 0.1% Triton X-100
Blocking
- Agent
- Milk
- Concentration
- 4%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Botch [N116/14R]
- Source
- Addgene
- Catalog Number
- 191818
- Species
- Mouse
- Gene / Target
- Chac1
- Concentration
- 10 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
- Source
- Thermo Fisher
- Catalog Number
- A21137
- Concentration
- 1:2500
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
COS cells were transfected with an antigen plasmid expressing Myc-tagged Botch.
- Protocol
- Trimmer lab immunocytochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)