Western Blot for Anti-Biotin [IPI-M33]
Addgene #218102
- Data Submitted By
- Zachary Anderson , Haisun Zhu , Travis Riedel , Deborah Moshinsky and Rob Meijers
- Lab Name
- Institute for Protein Innovation (IPI)
- Submission Date
- April 29, 2024
- Publication Date
- May 24, 2024 (modified September 23, 2024)
- DOI
- https://doi.org/10.57733/addgene.y5qsgp
- Abstract
- Experimental results for Anti-Biotin [IPI-M33] (Addgene Antibody 218102) in a western blot assay with a human sample.
- Citation
-
Western Blot for Anti-Biotin [IPI-M33]. Anderson Z, Zhu H, Riedel T, Moshinsky D, Meijers R 2024. Addgene Report, https://doi.org/10.57733/addgene.y5qsgp
![Recombinant biotinylated hLox1 protein was used as a positive control (+Biotin), and lysate from Expi293 cells transfected with Fab alone was used as the negative (Neg. control). Samples were separated by SDS-PAGE and immunoblotted against Anti-Biotin [IPI-M33].](https://datahub-media.addgene.org/report-images/y5qsgp/WB_Anti-Biotin_IPI-M33.png)
Image attribution: Institute for Protein Innovation (IPI)
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Biotin [IPI-M33]
Addgene #218102 -
IRDye 800CW Goat anti-Rabbit IgG
LICORbio #926-32211
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- Expi293
- Additional Information
- Recombinant biotinylated hLox1 protein was used as a positive control (+Biotin). Expi293 cells were transfected with Fab alone (Neg. control).
Buffer
- Name
- PBS-T
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 30 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Biotin [IPI-M33]
- Source
- Addgene
- Catalog Number
- 218102
- Species
- Rabbit
- Gene / Target
- Biotin
- Concentration
- 1 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- IRDye 800CW Goat anti-Rabbit IgG
- Source
- LICORbio
- Catalog Number
- 926-32211
- Species
- Goat
- Concentration
- 1:20,000
- Time
- 30 min
- Temperature
- Room Temperature
Additional Information
- Detection Method
- Fluorescence
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Strong signal for target