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Anti-BAF53b [N332B/15R] in Western Blot (Rat)

Hybridoma

Data Submitted By
James Trimmer
Lab Name
UC Davis/NIH NeuroMab Facility
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified March 18, 2024)
Abstract
Experimental results for Anti-BAF53b [N332B/15R] (hybridoma-derived) in a western blot assay with a rat sample.
Tissue and transfected cell immunoblot: extracts of rat brain low speed pellet (RBLSP) and COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N332B/15 (left) and N52A/42 (right) TC supe.
Caption: Tissue and transfected cell immunoblot: extracts of rat brain low speed pellet (RBLSP) and COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N332B/15 (left) and N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility
Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Caption: Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody
Anti-BAF53b [N332B/15R]
Addgene #184200

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain

Primary Antibody

Name
Anti-BAF53b [N332B/15R]
Species
Mouse
Gene / Target
Actl6b

Additional Information

Additional Information

NeuroMab hybridoma clone datasheet

Results

Result
Pass (The antibody worked under these conditions)