Return to Antibody Results

Anti-GFP [N86/38.1R] in Western Blot (Human)

Addgene #180084

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 28, 2024)
Abstract: Experimental results for Anti-GFP [N86/38.1R] (Addgene Antibody 180084) in a western blot assay with a human sample.

HEK293T cells were left untreated or transiently transfected with CAG-GFP (+GFP). 72h later cells were harvested, lysed, separated by denaturing SDS-PAGE and immunoblotted against Anti-GFP [N86/38.1R]. — Caption: HEK293T cells were left untreated or transiently transfected with CAG-GFP (+GFP). 72h later cells were harvested, lysed, separated by denaturing SDS-PAGE and immunoblotted against Anti-GFP [N86/38.1R].
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-GFP [N86/38.1R]
Addgene #180084; goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: HEK293T

Buffer

Name: TBS-T

Blocking

Agent: Milk
Concentration: 5%
Time: 60 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-GFP [N86/38.1R]
Source: Addgene
Catalog Number: 180084
Species: Mouse
Concentration: 2.5 µg/mL
Time: overnight
Temperature: 4 °C

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Concentration: 0.2 µg/mL
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information: HEK293T cells were left untreated or transiently transfected with CAG-GFP. 72h later cells were harvested, lysed and separated by denaturing SDS-PAGE
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 27 kDa

Results

Result: Pass (The antibody worked under these conditions)