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Anti-Alpha-Tubulin [F2C] in Immunocytochemistry (Human)

Addgene #196559

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-Alpha-Tubulin [F2C] (Addgene Antibody 196559) in an immunocytochemistry assay with a human sample.

HeLa cells were untransfected or transiently transfected with plasmid 56450, EGFP-Tubulin-6 ands stained with Anti-Tubulin [F2C]. Tubulin signal (red) is visible in the untransfected cells and the signal intensity increases following transfection with GFP-Tubulin. — Caption: HeLa cells were untransfected or transiently transfected with plasmid 56450, EGFP-Tubulin-6 ands stained with Anti-Tubulin [F2C]. Tubulin signal (red) is visible in the untransfected cells and the signal intensity increases following transfection with GFP-Tubulin.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-Alpha-Tubulin [F2C]
Addgene #196559; goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Thermo Fisher #A-1105

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: HeLa

Buffer

Name: PBS

Fixative

Fixative: Formaldehyde/Paraformaldehyde

Permeabilization

Agent: 0.2% Triton X-100 in PBS
Time: 10 min
Temperature: Room Temperature

Blocking

Agent: BSA
Concentration: 5%
Time: 20 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-Alpha-Tubulin [F2C]
Source: Addgene
Catalog Number: 196559
Species: Mouse
Gene / Target: TUBA1A
Concentration: 0.1 µg/mL
Time: 90 min
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Source: Thermo Fisher
Catalog Number: A-1105
Concentration: 2 µg/mL
Time: 30 min
Temperature: Room Temperature

Additional Information

Additional Information: HeLa cells were untransfected or transiently transfected with 56450, EGFP-Tubulin-6, and stained 24 h later with Anti-Tubulin [F2C]
Protocol: Addgene immunocytochemistry protocol

Results

Result: Pass (The antibody worked under these conditions)