Anti-Alpha-Tubulin [F2C] - Chimeric in Immunocytochemistry (Human)
Addgene #198269
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Alpha-Tubulin [F2C] - Chimeric (Addgene Antibody 198269) in an immunocytochemistry assay with a human sample.
![HeLa cells were untransfected or transiently transfected with plasmid 56450, EGFP-Tubulin-6 and stained with Anti-Tubulin [F2C].](https://datahub-media.addgene.org/report-images/w02878/198269-Immunohistochemistry-2023-04-03_145819.331008.png)
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Alpha-Tubulin [F2C] - Chimeric
Addgene #198269 -
Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Thermo Fisher #A-11012
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HeLa
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization
- Agent
- 0.2% Triton X-100 in PBS
- Time
- 10 min
- Temperature
- Room Temperature
Blocking
- Agent
- BSA
- Concentration
- 5%
- Time
- 20 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Alpha-Tubulin [F2C] - Chimeric
- Source
- Addgene
- Catalog Number
- 198269
- Species
- Rabbit
- Gene / Target
- TUBA1A
- Concentration
- 0.1 µg/mL
- Time
- 90 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
- Source
- Thermo Fisher
- Catalog Number
- A-11012
- Concentration
- 2 µg/mL
- Time
- 30 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
HeLa cells were untransfected or transiently transfected with 56450, EGFP-Tubulin-6, and stained 24 h later with Anti-Tubulin [F2C]
- Protocol
- Addgene immunocytochemistry protocol
Results
- Result
- Pass (The antibody worked under these conditions)