Anti-Neuroligin-1 [N97A/31R] in Western Blot (Mouse)

Hybridoma

Data Submitted By: Marc Bolliger and Tom Sudhof
Lab Name: Marc Bolliger and Tom Sudhof, Stanford
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-Neuroligin-1 [N97A/31R] (hybridoma-derived) in a western blot assay with a mouse sample.

Immunoblot against crude membranes from adult mouse brain (MBM), extracts of COS cells transiently transfected with Neuroligin-1, -2, -3, -4 or -4* plasmid and extracts of brains from wild-type (WT) and Neuroligin-1 knockout (KO) mice probed with N97A/31 TC supe. — Caption: Immunoblot against crude membranes from adult mouse brain (MBM), extracts of COS cells transiently transfected with Neuroligin-1, -2, -3, -4 or -4* plasmid and extracts of brains from wild-type (WT) and Neuroligin-1 knockout (KO) mice probed with N97A/31 TC supe.
Image attribution: Marc Bolliger and Tom Sudhof, Stanford

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-Neuroligin-1 [N97A/31R]
Addgene #180094

Materials & Methods

Sample

Species: Mouse
Cell / Tissue Type: Brain membranes

Primary Antibody

Name: Anti-Neuroligin-1 [N97A/31R]
Species: Mouse
Gene / Target: Nlgn1

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes