Anti-PSD-95 [K28/43] - Chimeric in Western Blot (Rat)
Addgene #196561
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified March 29, 2024)
- Abstract
- Experimental results for Anti-PSD-95 [K28/43] - Chimeric (Addgene Antibody 196561) in a western blot assay with a rat sample.
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-PSD-95 [K28/43] - Chimeric
Addgene #196561 -
Goat anti-Rabbit IgG Fc, HRP
Thermo Fisher #A16116
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Total Amount
- 30 µg
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-PSD-95 [K28/43] - Chimeric
- Source
- Addgene
- Catalog Number
- 196561
- Species
- Rabbit
- Gene / Target
- DLG4
- Concentration
- 1 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-Rabbit IgG Fc, HRP
- Source
- Thermo Fisher
- Catalog Number
- A16116
- Species
- Rabbit
- Concentration
- unknown
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Goat anti-mouse IgG (H+L) HRP (Thermo Fisher, catalog number A16078) used to detect Anti-PSD-95 recombinant mouse antibody (180082)
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 95-110 kDa (varies with cell background due to phosphorylation)
Results
- Result
- Pass (The antibody worked under these conditions)